Journal of In Vitro Fertilization

Case Report Pages 30-32

Sterile Water Test Can Shows Signs of Fertilisation and Further Growth of Embryo- A Case Report

Authors: G. Shiva Krishna and Sree Durga Patchava

Abstract: Structural integrity and function of the plasma membrane of the cell defines the viability of spermatozoa. HOS (Hypo-Osmotic Swelling) test is considered as Benchmark test for checking the vitality of spermatozoa. The constituents of HOS test are 0.735g of sodium citrate dihydrate and 1.351g of D-fructose in 100 ml of purified water has an osmolarity of 150 mOsm. The basic principle of HOS test is spermatozoa when subjected to HOS solution, Sperm tails expand and coils based on plasma membrane integrity. Coiled tails are considered as vital spermatozoa. Spermatozoa which do not show any coiling due to loss of plasma membrane integrity are considered as dead spermatozoa.

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